serum adiponectin concentration  (R&D Systems)

Journal: Diabetology & Metabolic Syndrome

Article Title: Adiponectin complexes composition in Japanese-Brazilians regarding their glucose tolerance status

doi: 10.1186/1758-5996-5-20

Figure Lengend Snippet: Representative immunoblot from serum of age and sex matched subjects with NGT (lanes 1 and 2) and DM (lanes 3 and 4). Band at > 300 kDa represents high molecular weight (HMW) adiponectin, at ~180 kDa represents middle molecular weight (MMW) adiponectin and at ~70 kDa represents low molecular weight (LMW) adiponectin.

Article Snippet: Serum total adiponectin concentrations were measured by radio-immunoassay according to manufacter’s instructions (Linco Research, St. Charles, MI, USA) in specimens that had been stored at −20°C.

Techniques: Western Blot, Molecular Weight

Journal: Diabetology & Metabolic Syndrome

Article Title: Adiponectin complexes composition in Japanese-Brazilians regarding their glucose tolerance status

doi: 10.1186/1758-5996-5-20

Figure Lengend Snippet: Serum concentrations of total, low molecular weight (LMW), middle molecular weight (MMW) and high molecular weight (HMW) adiponectin in individuals with normal glucose tolerance (NGT) or diabetes mellitus (DM). The box displays the median and interquartile range (25th-75th percentile) and the whiskers display the 10th and 90th values.

Article Snippet: Serum total adiponectin concentrations were measured by radio-immunoassay according to manufacter’s instructions (Linco Research, St. Charles, MI, USA) in specimens that had been stored at −20°C.

Techniques: Molecular Weight

Journal: Diabetology & Metabolic Syndrome

Article Title: Adiponectin complexes composition in Japanese-Brazilians regarding their glucose tolerance status

doi: 10.1186/1758-5996-5-20

Figure Lengend Snippet: Percentual distribution of low molecular weight (LMW), middle molecular weight (MMW) and high molecular weight (HMW) adiponectin in individuals with normal glucose tolerance (NGT) or diabetes mellitus (DM).

Article Snippet: Serum total adiponectin concentrations were measured by radio-immunoassay according to manufacter’s instructions (Linco Research, St. Charles, MI, USA) in specimens that had been stored at −20°C.

Techniques: Molecular Weight

Journal: Diabetology & Metabolic Syndrome

Article Title: Adiponectin complexes composition in Japanese-Brazilians regarding their glucose tolerance status

doi: 10.1186/1758-5996-5-20

Figure Lengend Snippet: Odds ratio (OR) and 95% confidence interval (CI) for diabetes mellitus according to biological variables

Article Snippet: Serum total adiponectin concentrations were measured by radio-immunoassay according to manufacter’s instructions (Linco Research, St. Charles, MI, USA) in specimens that had been stored at −20°C.

Techniques:

Journal: Redox Biology

Article Title: Loss of Selenov predisposes mice to extra fat accumulation and attenuated energy expenditure

doi: 10.1016/j.redox.2021.102048

Figure Lengend Snippet: Effects of Selenov knockout on body weight, tissue fat accumulation, adipocyte histology, and serum lipid profiles and related biomarkers in male mice fed a normal-fat (control) or a high-fat diet from 2- to 6-month old of age. (A) Body weight (n = 10). (B) Upper panel images: magnetic resonance analysis of total body fat mass. Both the vertical (top) and cross (bottom) sections showed increased fat (red color) accumulations by KO and high-fat diet. Lower bar graphs: weights of total fat and individual adipose tissues (n = 10). (C) H&E staining of paraffin-embedded EWAT sections: adipocyte hypertrophy and enlarged lipid droplets by the KO and high-fat diet, scale bar, 20 μm. The image was a representative of five sets of data. (D) Serum concentrations of TC, TG, NEFA, leptin, TNF α , IL6, Mcp1, and adiponectin (n = 6). All graphic data are mean ± SE. Means without sharing a common superscript letter are different (P < 0.05). Abbreviations: BAT, brown adipose tissue; EWAT, epididymis white adipose tissue; HF, high-fat diet; IL6, interleukin 6; KO, knockout; Mcp1, monocyte chemoattractant protein-1; NEFA, non-esterified fat acid; NF, normal-fat diet; TC, total cholesterol; TG, total triglyceride; TNF α , tumor necrosis factor alpha; WT, wild-type. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Respective commercial kits were also used to determine serum concentrations of adiponectin and leptin (Crystal Chem, Downers Grove, IL, USA), interleukin 6 (IL-6) and tumor necrosis factor α (TNFα) (eBioscience, San Diego, CA, USA), and monocyte chemoattractant protein-1 (Mcp1) (BioLengend, San Diego, CA, USA).

Techniques: Knock-Out, Staining